LOCUS       jsSi1906_RMCE_II        6828 bp    DNA        linear       10-MAY-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..711
                     /locus_tag="Mos insertion region"
                     /label="Mos insertion region"
                     /ApEinfo_label="Mos insertion region"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5410..5443)
                     /locus_tag="FRT3"
                     /label="FRT3"
                     /ApEinfo_label="FRT3"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    726..759
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5446..6828
                     /locus_tag="Mos insertion region(1)"
                     /label="Mos insertion region(1)"
                     /ApEinfo_label="Mos insertion region"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    665..666
                     /locus_tag="ttTi5605 insertion site"
                     /label="ttTi5605 insertion site"
                     /ApEinfo_label="ttTi5605 insertion site"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     204..225
                     /locus_tag="NMoligo 3887"
                     /label="NMoligo 3887"
                     /ApEinfo_label="NMoligo 3887"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    810..1785
                     /locus_tag="pMYO-2"
                     /label="pMYO-2"
                     /ApEinfo_label="pMYO-2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1932..1982
                     /locus_tag="Synthetic Intron 1"
                     /label="Synthetic Intron 1"
                     /ApEinfo_label="Synthetic Intron 1"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(1826..1931,1983..2111,2163..2293,2345..2569,
                     2621..2734)
                     /locus_tag="NeonGreen_Cel"
                     /label="NeonGreen_Cel"
                     /ApEinfo_label="NeonGreen_Cel"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5602..5622)
                     /locus_tag="NMoligo 3888"
                     /label="NMoligo 3888"
                     /ApEinfo_label="NMoligo 3888"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     UTR             4959..5399
                     /locus_tag="glh-2 3' UTR"
                     /label="glh-2 3' UTR"
                     /ApEinfo_label="glh-2 3' UTR"
                     /ApEinfo_fwdcolor="#ffc0cb"
                     /ApEinfo_revcolor="#ffc0cb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          4959..5089
                     /locus_tag="sequenced 3834"
                     /label="sequenced 3834"
                     /ApEinfo_label="sequenced 3834"
                     /ApEinfo_fwdcolor="#212121"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden=""
     misc_feature    2570..2620
                     /locus_tag="Synthetic Intron 4"
                     /label="Synthetic Intron 4"
                     /ApEinfo_label="Synthetic Intron 4"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    1789..1822
                     /locus_tag="FRT10"
                     /label="FRT10"
                     /ApEinfo_label="FRT10"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5315..5316
                     /locus_tag="84 bp deletion"
                     /label="84 bp deletion"
                     /ApEinfo_label="84 bp deletion"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2294..2344
                     /locus_tag="Synthetic Intron 3"
                     /label="Synthetic Intron 3"
                     /ApEinfo_label="Synthetic Intron 3"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2112..2162
                     /locus_tag="Synthetic Intron 2"
                     /label="Synthetic Intron 2"
                     /ApEinfo_label="Synthetic Intron 2"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           2741..3070
                     /locus_tag="tbb-2 3'UTR"
                     /label="tbb-2 3'UTR"
                     /ApEinfo_label="tbb-2 3'UTR"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3582..4952
                     /locus_tag="FLP D5"
                     /label="FLP D5"
                     /ApEinfo_label="FLP D5"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3086..3571
                     /locus_tag="mex-5p"
                     /label="mex-5p"
                     /ApEinfo_label="mex-5p"
                     /ApEinfo_fwdcolor="#ff7d78"
                     /ApEinfo_revcolor="#ff7d78"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5158..5158
                     /locus_tag="poly A site"
                     /label="poly A site"
                     /ApEinfo_label="poly A site"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ATTTCGTGGT TTATATCCTC TGAACTGGTA CCTCAAAAAC ATCGTAGTAT TGCTCAATCT
       61 GTGGCATATT CAATGAATAC TGTAATGGTG GTTATCAGTA CATTTACAGT ATTACCTCTA
      121 TATTCCGTTA TTGGTAGTTA TGCTTTCCTT ATTCTATATT CAATTCCTTC ATTTATAAGT
      181 ATGCTTATAT TATTTCGCTA CTTACCGGAA ACCAAAGGAC GAGAGATTCA TGATATTGTG
      241 AATGAACTTA AAAAAAAATG AAAACCTTTG AAAACGTTCA TACGGCCGAA ATTTTACTTG
      301 ATCGAGCCAA TTGAACTTTT GTTAAGACTT ACAGAAAAGT GAGAGATTTT GTTACAAAAA
      361 AGTGAGAAAA AAGCGAAAAT GAACAGGAAA AAGAAGAAAA ATACACCCGA AGTATCATAT
      421 AAAAATTAAA AAGATTGTTT GACCTGGCGG AACTTTTTTG CAAAAACTCT GAATATCCAT
      481 GGCACATGCT TCGTGCAAAA CAGGATCCAA ATGAATATCA ACAAGAGATT CTTGAAGACG
      541 ACGAGCCACT TGCTCAGAGC ATTGCTTATC TCGAATGAGA CCCTTGTTAA AATCATTTTT
      601 CAAACATTAT ATGACATTGT CGAAATGTCC TCCTGATTCC ATGATGGTAG CAAACTCACT
      661 TCGTGTAAGT GCAAGTAAGA TCAGTGTTTG TTTCGATATC AGTCTGTTTC GAAGgtaagt
      721 ttaaaATAAC TTCGTATAGC ATACATTATA CGAAGTTATA CGggcgaatt ggagctccac
      781 cgcggtggcg gccgctctag aaCTAGTGGc attttatatc tgagtagtat cctttgcttt
      841 aaatgtccat aaaactaatt ttataatcaa taaaacaacg tttgtaaatc aactgagttt
      901 acaagtagag acattgaggg atactttcac tatgctaaag tgataatcga ccaaataata
      961 acttcacttt ggtatttatt cctgtcttat aaatgttatg tatgaattaa attcatatgc
     1021 atatggctca ctctgacaat ttttaataat cttccagatc aatattgact accgatgcgg
     1081 gtggtctttt gctttgaatt ctgctgaact ttacaccccg aacagcaatg tgtgcttcag
     1141 cctaaaaaaa agtaagtgtg ttaatcagtg cccccgattc ttcatttttt gcccctctct
     1201 cccgtttcgt cggcaaaaga agagaaaata aagataagtc tcaagatagg ttggtaatcg
     1261 ctaaagtggt tgtgtggata agagtagcaa aatggcagga ggagcacttt gcgcgcacac
     1321 actgtactca ttgttctgga taaaattctc tcgttgtttg ccgtcggatg tctgcctctc
     1381 tgcattgagc cggcttcttc actatcttta gttaacctaa aatgccgttt cttttctcgt
     1441 atccccacta tcccgttgag gttctctgct ctcttcgctc cctaccgcca gcgagcaact
     1501 atccgtgggg gcgccttgct cggaagatgg gggggaagaa agaagatttt tgctatttgc
     1561 acttgagaaa gagacttttc ctgcgtcgat ggtCagagaa cagtgtgcag acacttttca
     1621 gctacctaga attacaattg gatatccccg cctcccaatc cacccaccca gggaaaaaga
     1681 agggctcgcc gaaaatcaaa gttatctcca ggctcgcgca tcccaccgag cggttgactt
     1741 ctctccacca cttttcattt taaccctcga tcgtcagaca cagaaGCGGA AGTTCCTATT
     1801 CactagaatG TATAGGAACT TCAAAATGGT GTCGAAGGGA GAAGAGGATA ACATGGCTTC
     1861 ACTCCCAGCT ACACACGAAC TCCACATCTT CGGATCGATC AACGGAGTGG ATTTCGATAT
     1921 GGTCGGACAA Ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc
     1981 agGAACTGGA AACCCAAACG ATGGATACGA GGAACTCAAC CTCAAGTCGA CAAAGGGAGA
     2041 TCTGCAATTC TCGCCATGGA TTCTCGTGCC ACACATCGGA TACGGATTCC ACCAATACCT
     2101 CCCATACCCA Ggtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc
     2161 agATGGAATG TCACCATTCC AAGCTGCCAT GGTGGATGGA TCGGGATACC AAGTTCACCG
     2221 AACAATGCAA TTCGAGGATG GAGCCTCGCT CACAGTGAAC TACCGATACA CATACGAGGG
     2281 ATCGCACATC AAGgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
     2341 tcagGGAGAG GCTCAAGTTA AGGGAACAGG ATTCCCAGCT GATGGACCAG TGATGACAAA
     2401 CTCACTCACA GCTGCTGATT GGTGCCGATC GAAAAAGACA TACCCAAATG ATAAGACAAT
     2461 CATCTCGACA TTCAAGTGGT CGTACACTAC TGGAAACGGA AAGCGATACC GATCGACAGC
     2521 CCGAACAACA TACACATTCG CTAAGCCAAT GGCCGCCAAC TACCTCAAGg taagtttaaa
     2581 catgatttta ctaactaact aatctgattt aaattttcag AATCAACCAA TGTACGTGTT
     2641 CCGAAAGACA GAACTCAAGC ACTCAAAGAC AGAGCTGAAC TTCAAAGAGT GGCAAAAGGC
     2701 CTTCACAGAT GTGATGGGAA TGGATGAACT CTACAAGTAA taaatgcaaa atcctttcaa
     2761 gcattccctt cttctctatc actcttcttt ctttttgtca aaaaattctc tcgctaattt
     2821 atttgctttt ttaatgttat tattttatga ctttttatag tcactgaaaa gtttgcatct
     2881 gagtgaagtg aatgctatca aaatgtgatt ctgtctgatg tactttcaca atctctcttc
     2941 aattccattt tgaagtgctt taaacccgaa aggttgagaa aaatgcgagc gctcaaatat
     3001 ttgtattgtg ttcgttgagt gacccaacaa aaagaggaaa ctttattgtg ccgccaagaa
     3061 aaaagtctca GTAGCatGCG GCCGCATATC AGTTTTTAAA AAATTaaacc ataaaacaaa
     3121 taatataacc caatttttac atcaaaccac aagaaaaaaa tacatttggg cccacggata
     3181 aagaaattaa aaaaatacat tttttaaagg cgcaccgaat taaaattcat ttgggtctta
     3241 ccgcgtatac cgtactccgt ttgtttgatc atttttgtca gcgctggcgg ttgttttttc
     3301 atttcatttc tgcttcaaag acgttttctc gaataatttt tcgtttattc tcttttttaa
     3361 aattaatttc tagccgtaaa tgttataaat tcacccattt aacgcaaatt tcatggtaat
     3421 ctcatggaaa aatgcagttt ctttgttaaa gaaagcttaa atagcaaaaa ttccccgact
     3481 ttccccaaaa tcctgctcga ttttccgttt tctcattgta ttctctctta attaatttta
     3541 tcgataatca aTTGAATGTT TCAGACAGAG AGGTACCAAA AATGCCACAA TTCGACATTC
     3601 TCTGTAAGAC ACCACCAAAA GTCCTCGTCC GTCAATTCGT CGAGCGTTTC GAGCGTCCAT
     3661 CCGGAGAGAA GATCGCCCTC TGCGCCGCCG AGCTCACCTA CCTCTGCTGG ATGATCACCC
     3721 ACAACGGAAC CGCCATCAAG CGTGCCACCT TCATGTCCTA CAACACCATC ATCTCCAACT
     3781 CCCTCTCCTT CGACATCGTC AACAAGTCCC TCCAATTCAA GTACAAGACC CAAAAGGCCA
     3841 CCATCCTCGA GGCCTCCCTC AAGgtaagtt taaacatata tatactaact aaccctgatt
     3901 atttaaattt tcagAAGCTC ATCCCAGCCT GGGAGTTCAC CATCATCCCA TACTACGGAC
     3961 AAAAGCACCA ATCCGACATC ACCGACATCG TCTCCTCCCT CCAACTCCAA TTCGAGTCCT
     4021 CCGAGGAGGC CGACAAGGGA AACTCCCACT CCAAGAAGAT GCTCAAGGCC CTCCTCTCCG
     4081 AGGGAGAGTC CATCTGGGAG ATCACCGAGA AGATCCTCAA CTCCTTCGAG TACACCTCCC
     4141 GTTTCACCAA GACCAAGACC CTCTACCAAT TCCTCTTCCT CGCCACCTTC ATCAACTGCG
     4201 GACGTTTCTC CGACATCAAG AACGTCGACC CAAAGTCCTT CAAGCTCGTC CAAAACAAGT
     4261 ACCTCGGAGT CATCATCCAA TGCCTCGTCA CCGAGACCAA GACCTCCGTC TCCCGTCACA
     4321 TCTACTTCTT CTCCGCCCGT GGACGTATCG ACCCACTCGT CTACCTCGAC GAGTTCCTCC
     4381 GTAACTCCGA GCCAGTCCTC AAGCGTGTCA ACCGTACCGG AAACTCCTCC TCCAACAAGC
     4441 AAGAGTACCA ACTCCTCAAG GACAACCTCG TCCGTTCCTA CAACAAGGCC CTCAAGAAGA
     4501 ACGCCCCATA CTCCATCTTC GCCATCAAGA ACGGACCAAA GTCCCACATC GGACGTCACC
     4561 TCATGACCTC CTTCCTCTCC ATGAAGgtaa gtttaaacat gattttacta actaactaat
     4621 ctgatttaaa ttttcagGGA CTCACCGAGC TCACCAACGT CGTCGGAAAC TGGTCCGACA
     4681 AGCGTGCCTC CGCCGTCGCC CGTACCACCT ACACCCACCA AATCACCGCC ATCCCAGACC
     4741 ACTACTTCGC CCTCGTCTCC CGTTACTACG CCTACGACCC AATCTCCAAG GAGATGATCG
     4801 CCCTCAAGGA CGAGACCAAC CCAATCGAGG AGTGGCAACA CATCGAGCAA CTCAAGGGAT
     4861 CCGCCGAGGG ATCCATCCGT TACCCAGCCT GGAACGGAAT CATCTCCCAA GAGGTCCTCG
     4921 ACTACCTCTC CTCCTACATC AACCGTCGTA TCACGTAGAT GTATCTGCAT ATCCTTCAAA
     4981 TGTTGTCCAT ATTGTATCAG TAAATTATAA TGCCCCCTTT TTATATATTC TCCTTATCAT
     5041 GTAAATGTTC TATTGATTTT TTGCTGGTGT AAAACGTTTT TATAGTAAAT CCTCTCCACA
     5101 GTGAATTTGT TAAATTGTAT CAAAACAAGA CATTGATTAA ATTTGTTCGA GACTTGTGGA
     5161 TTGTAAAATA TTTTGCAGAA AATGGCAATA TTCATTTAGA AACATTACTT CCATAATAGG
     5221 AAAAACTATT TATGAGTTTT CATGTGCCTA TGCAAGTTCC CACTCTGTGT GAATTTCATC
     5281 ATACAAATTG AGCAAACATA AGGTTTATGT CCAGTGTGTG TTCTGAAATA ATGAAAAATA
     5341 GTCTTGCAAT ATGTTTCTAC TATTCGAGAA AAAATGTAAT TTTTTTTCTT ATGGTAACCc
     5401 gGTAcccggG AAGTTCCTAT ACtatttgaa GAATAGGAAC TTCATGTAAC GGTCTTCTGT
     5461 ATAACTACAA AAAAGAATAA AAAACCGTAT CACAACTGAG AAAAAAGACA AAACTTATTG
     5521 AACAGTGTTC CTTTCAAAAA ATTATTTTGT AAAAAATCGA GTTTTTCTCC AAAATAACGC
     5581 ACGCAATCAA AAACAAGAAA ACTAACGTGT TCTCCTGGGC GTGGAACTCC TTATCAAGAT
     5641 TACCATTATT GTCTGTCAGA ATTGATATTT CAATTATTTC ATATCTAGAC AAACCCGGGT
     5701 GTCTTGCATT TATTAGTACT GTGAGTTTAC ATACAAACGA GGCTTACTCA GATAACATAG
     5761 AGTCACCTCT AATTAGCAAA AACCCATTTC TAATTAGCTA TTTCGACCAG TTCTAAAGTC
     5821 ACCGTAATTA GAAATGGTTT TTGTAGCTTT TCTAATTAGA AATGACCCCG AGATAAGTGA
     5881 AATATGGCAT TTTCTGGGTT TTCTAGTAAG AAATGATCCC GCACGTAGTG ATTCCGCGTT
     5941 ACAAGCCAAA TTCAAATTAA AAAAGTATAT TACACAATTC AAGTTACATT ATTTTCAGGA
     6001 AACAATGGCG AAGAGCAAAG CAGAGATTCA AAAGGAGGAA AGAGACAACA GATATGAACG
     6061 AAGACTTCTT GAAAAGAGAA AGAGACATGA GGTGAGTAAC CATGATAATA AATAATCAAG
     6121 TAAGGGCTAT AGATTTATAA ATTCTGGAGT TAACAGTTTC GAAAAAAAGT AGTTTTCATA
     6181 TGTGCGAAGG AATATCAAAA ATAGGGGAAT AGCCTAAACG AGTGAGAAAA TTAAATGTTT
     6241 GTTATTTTTT CAAAAATGTC TGCAGGGCCG TTATCCTTTC AAATGCACTC TATCAAACAT
     6301 AGATCATATC CATCATATCT TATCCAGAAT TTATATCATA TCCAAGATTG AGAGCGTTTC
     6361 AAGGAAAATC TCAAACAACC ATACAATTTT GTAGATCGAC AACAAGGCCT CCACTTCTTC
     6421 ATCGATGGCC TCTGCAGCAC CTGCACCACC AAGTTTGTAT TTTTCATATT AATATAAAGT
     6481 GAGCCACGCA TAGTCGTTTT TAGGATTTTT GCCAGATCGA TGTCGCGCAA ACAGACTGTT
     6541 TTTTTTTCTT TGAAAGCCGT GCGTGACATG GGCCACGGAT TGTAACGCCG CGCTGGATGG
     6601 ATAAAAATTT ATTCAGTTTT TTAGCCTTTA CAAAATATGG TTCGACTACT TTCTTTATTT
     6661 TTACATTTTT ATAGGTGCTC ATTGAGCTGA GATATTTTGG GTATAATCGG AAAATCGCTG
     6721 ATTTTGGTCA TTTTTCTGCA CTAAAGATTA CACAAAAAGT TCAGTATTTA GATTCATTGT
     6781 TGAAAATTGG GGATGATCTA CAGATACATA TCGCCGTGTT CAAAAACA 
//
